Mechanistic aspects of ascorbate inhibition of human immunodeficiency virus.Chem Biol Interact 1994 Jun;91(2-3):207-15 (ISSN: 0009-2797) Harakeh S; Niedzwiecki A; Jariwalla RJ We have investigated the molecular basis of the inhibitory effect of
ascorbate (vitamin C) on human immunodeficiency virus (HIV) expression
in unstimulated chronically infected and reporter cell lines. Comparison
of intracellular HIV RNA and protein patterns of ascorbate-treated cells
with corresponding patterns of untreated controls, did not show significant
differences in the synthesis or processing of individual viral RNA and
polypeptides, indicating that the inhibitory effect of ascorbate is not
directed at steps of viral transcription or translation. Enzyme assays
on cell extracts showed that the activity of an HIV LTR-directed reporter
protein made in ascorbate-treated cells was reduced to approximately 11%
relative to that of untreated control. These results, combined with previous
observations on the suppression of HIV RT activity, are consistent with
a mechanism of action in which ascorbate exerts a posttranslational inhibitory
effect on HIV by causing impairment of enzymatic activity. |
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